For detailed information on services and prices, please use our contact sheet or call us directly (phone: +49 (0)89-8955-7840).
We will gladly assist you with our long standing experience in Real-Time PCR.
For recommendations on sample collection, storage and shipment, please refer to our Sample Submission Guidelines - qPCR Analysis.
Real-time PCR becomes especially challenging whenever the amount of starting material is limited, e.g. when analyzing biopsies or samples generated by laser-capture microdissection (LCM). IMGM applies the latest pre-amplification techniques to allow for successful analysis of multiple target genes in such demanding materials.
The following service options are available for Real-Time PCR:
- RNA isolation
- RNA quality control (Agilent Bioanalyzer)
- Pre-amplification of limited amounts of RNA or cDNA
- Assay selection and assay design
- Selection of endogenous controls (housekeeping genes)
- Reverse transcription
- Quantitative Real-Time PCR (AB7900HT)
- 96-well, 384-well or TaqManŽ Array (384) format
- Data analysis by ΔΔCt method
- Reporting
IMGM Laboratories offers a large collection of pathway- and disease-specific Gene Signature Panels (Applied Biosystems).
For detailed lists of currently available Gene Signature Panels, follow the links below:
To customize your own TaqManŽ Array, you decide on your set of genes and select from available plate layouts.
Please contact us for a detailed discussion on optimal formats for your gene expression project.
For information on DNA-based Real-Time PCR, please click here.
