• Whole genome sequencing is a powerful technique that generates comprehensive information about the entire genome. At IMGM we offer de-novo assembly, generating a reference genome for your organism of choice. This might be a novel organism or polishing the genome of known organisms. Whole-genome re-sequencing enables us to detect DNA variations in any part of the genome at a reasonable price by alignment of sequencing data to a reference genome. This includes variants that might be missed with targeted approaches. Variations that can be detected range from small variants like SNPs and short indels, to larger variants such as intra-chromosomal translocations or copy number variations. This information can be used in disease research, diagnostics and personalized medicine, as well as in basic genomics research or agricultural research.
• Targeted gene sequencing utilizes gene panels or certain target regions containing a select set of genes/regions that are known to have association with the phenotype of interest. This technique offers a resource efficient way to investigate a specific part of the genome.

The exome consists of the protein coding regions of the genome. It represents only a minor part of the whole genome while harboring most of the genetic variants that are known to have a large effect on the phenotype. Exome sequencing allows for a cost-effective and efficient analysis of the most functionally significant portions of the genome and has revolutionized the field of genetics and genomics, aiding in the identification of disease-causing mutations, understanding genetic diversity, and uncovering potential therapeutic targets for various disorders. Excluding non-coding regions, enables the identification of genetic variants and mutations that are more likely to have functional implications.

The transcriptome represents all RNA transcribed by an organism or a population of cells at a specific biological state. Transcriptome sequencing (RNA-Seq) utilizes next generation sequencing technologies to gain comprehensive insight into cellular functions, identifying disease mechanisms, and discovering potential therapeutic targets. Transcriptome sequencing offers insights into coding and non-coding RNAs, including novel transcripts and splice variants.

At IMGM, we offer various solutions for RNA-Sequencing (small RNA, mRNA and total RNA), enabling researchers to explore differences in gene expression between conditions and to study regulatory networks. Ultimately, transcriptome sequencing transforms raw data into valuable biological insights through bioinformatics analyses.

Cell populations usually exist as a heterogeneous ensemble of cell types at different stages of differentiation. Single-cell RNA sequencing is the key to deciphering these challenging samples. Using microfluidic techniques, the mRNA transcripts of each cell are individually labeled with barcodes and UMIs and sequenced after subsequent library preparation.

As a certified service provider for 10x Genomics 3′ Gene Expression and 5′ Immune Profiling workflow, IMGM is at the forefront of single cell analysis with an experienced team of PhD-level scientists.

Our services include:

In addition, we are the ideal contact for your spatial transcriptomics project. We manage the entire workflow from sample to data transfer in close coordination with our cooperation partner Indivumed Services.

Unlock the full potential of your samples with IMGM and . We offer sequencing of Illumina compatible Ready-To-Load libraries. Trust us with sequencing your in-house libraries and discover the IMGM difference:

• Benefit from the latest next-generation sequencing (NGS) technology and our extensive expertise in sample handling.
• Choose from a wide range of sequencing options, specifically tailored for your project.
• Accurate determination of library concentration and appropriate sequencing dilution.
• Detailed sequencing report.

Secure data delivery and personal project management make it easy to collaborate with us and stay informed about your progress. Entrust IMGM with your next NGS project.

Gene expression analysis offers information about the profile and quantity of functional RNA species or protein products in an organism or population of cells at a certain biological state. As such, it provides insights into normal cellular processes, such as differentiation, as well as abnormal or pathological processes. IMGM Laboratories GmbH holds a certificate of Good Laboratory Practice (GLP) compliance for quantitative nucleic acid sequence detection issued by the Bavarian Health and Food Safety Authority.

Our expert team at IMGM uses state of the art PCR techniques to measure gene expression in a relative manner (relative quantification) or against a standard (absolute quantification):

ddPCR

Our Biorad QX200 digital droplet PCR system is based on nucleic acid amplification in oil droplets followed by a fluorescence-based end-point detection of positive droplets in comparison to negative droplets. Separated amplification in multitude droplet-reactions highly increases the precision of the signal, easily allows multiplex PCR and sensitively detects even small amounts of nucleic acid targets without the need of a standard curve. Even difficult samples like cell free DNA or FFPE material are accessible as the droplet approach reduces inhibitory effects. IMGM’s ddPCR service can be provided under GLP or GCP guidelines.

qPCR

At IMGM, we offer qPCR analysis on Life Technologies or Biorad thermocyclers using probe-based (e.g. TaqMan) or dye-based (e.g. SYBR Green) chemistry. We offer assay design and validation, absolute and relative quantification, and analysis of various RNA species including miRNAs and mRNAs.

The IMGM team can help you with designing your qPCR/ddPCR project perfectly. We generate first-class data and offer bioinformatics data analysis to provide you with high-value information about your samples:

• Assay design:
  • We support you in close communication to develop the appropriate assay you need.
  • Alternatively, we can use assays developed in your lab or commercially available assays.
  • We provide you with detailed study overview and time frames
• DNA and RNA extraction:
  • We use validated high-quality DNA and RNA extraction methods minimizing contaminations.
  • Nucleic acids are quantified with standardized methods (Nanodrop, Qubit, Bioanalyzer)
• Assay performance and data analysis:
  • Highly accurate ddPCR or qPCR detects your target of interest.
  • QC of each run to guarantee high quality raw data.
  • Additional data analysis is provided on request.
  • GLP- or GCP-compliant documentation is possible.

Genotyping in general, is the measurement of small genetic variations and their comparison between members of a species. Single nucleotide polymorphisms (SNPs), copy number variations (CNVs) and Insertions or Deletions (InDels) are among the most common types of genetic variations.

SNPs are single base pair mutations in the genome and have a vast range of uses such as research, medicine and pharmacogenetic studies. CNVs are a type of multiplication or deletion of a segment of the genome and are known for example to influence the phenotype of diseases. Insertions and deletions of short segments of the genome lead to polymorphisms and are used in phylogenetic studies for example.

Various technologies can be used for pharmacogenomics genotyping. One of them is real-time PCR – a low cost per sample and a fast method suitable for routine testing. Possible applications are:

• detect biologically important polymorphisms (e.g. Cytochrome P450) with roles in drug metabolism and transport
• Detection of the proportion of mutant cells in genetically modified organisms.
• Rare mutation detection e.g., to identify tumor cells.

Besides catalog qPCR and next generation sequencing solutions, IMGM offers professional assay designs, tailored to your specifications, a competitive price-benefit ratio, whether you have just a small or a high-throughput project. Our biomarker discovery service is rounded off by expert bioinformatics analyses that will also be adjusted to your request.

Accelerate your research with the best informatics strategy. IMGM provides a systems biology informatics toolkit to capture, query, and visualize your genomic data. We explore the variation between your samples (SNP, CNV) or integrate your expression data to Identify differentially expressed genes from RNA-Seq, Single Cell Sequencing, and other expression studies. Our scientists have decades of experience in biology and computer science and look forward to revealing the secrets of your data.